Brenton D. Hoffman
Associate Professor in the Department of Biomedical Engineering
The overall goal of my research program is to utilize an interdisciplinary approach to first advance the basic understanding of mechanotransduction on multiple scales and then use this knowledge to guide the development of new treatments for mechanosensitive diseases. Our work combines principles and techniques from protein engineering, molecular biology, soft matter physics, cell and developmental biology, biomaterials engineering, automated image analysis, and state of the art live cell microscopy. Specifically, we engineer and use biosensors that report the tension across specific proteins in living cells through changes in the color of light they emit. This technology enables dynamic measurements of proteins and sub-cellular structures that are under load. Unlike more traditional techniques that measure the entirety of cellular force output, the ability of these sensors to measure mechanical stress at the molecular level means they are innately compatible with concepts and approaches common in molecular biology and biophysics.
Appointments and Affiliations
- Associate Professor in the Department of Biomedical Engineering
- Assistant Professor in Cell Biology
- Ph.D. University of Pennsylvania, 2007
Focused on understanding, on a molecular level, how mechanical and chemical cues from the environment are detected, integrated, and manipulated by cells to dictate physiological and patho-physiological responses important in vascular biology.
Awards, Honors, and Distinctions:
- Scholars. Searle Scholars. 2013
- BME 302L: Fundamentals of Biomaterials and Biomechanics (AC or GE)
- BME 394: Projects in Biomedical Engineering (GE)
- BME 493: Projects in Biomedical Engineering (GE)
- BME 494: Projects in Biomedical Engineering (GE)
- BME 527: Cell Mechanics and Mechanotransduction (GE, BB, MC)
- BME 711S: Biological Engineering Seminar Series (CBIMMS and CBTE)
- BME 712S: Biological Engineering Seminar Series (CBIMMS and CBTE)
- BME 791: Graduate Independent Study
- BME 792: Continuation of Graduate Independent Study
- ME 717S: Biological Engineering Seminar Series (CBIMMS and CBTE)
- ME 718S: Biological Engineering Seminar Series (CBIMMS and CBTE)
- PHYSICS 493: Research Independent Study
- PHYSICS 495: Thesis Independent Study
- Hoffman, BD; Grashoff, C; Schwartz, MA, Dynamic molecular processes mediate cellular mechanotransduction, Nature, vol 475 no. 7356 (2011), pp. 316-323 [10.1038/nature10316] [abs].
- Grashoff, C; Hoffman, BD; Brenner, MD; Zhou, R; Parsons, M; Yang, MT; McLean, MA; Sligar, SG; Chen, CS; Ha, T; Schwartz, MA, Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics, Nature, vol 466 no. 7303 (2010), pp. 263-266 [10.1038/nature09198] [abs].
- Hoffman, BD; Massiera, G; Citters, KMV; Crocker, JC, The consensus mechanics of cultured mammalian cells, Proceedings of the National Academy of Sciences of the United States of America, vol 103 no. 27 (2006), pp. 10259-10264 [10.1073/pnas.0510348103] [abs].