Control over the presence of biologics (e.g. stem cells, growth factors) is a common theme in natural tissue formation, and also an emerging theme in functional tissue engineering strategies. However, a persistent challenge in tissue engineering approaches has been to effectively incorporate biologics into tissue engineering devices while maintaining optimal physical and chemical properties of the device. In particular, there is often a design trade-off between effective biologic delivery and optimal scaffold physical/chemical properties. This talk will present a series of coating strategies we have used to deliver genes, growth factors, and stem cells from tissue engineering devices. Fundamental mechanisms of affinity binding are used to incorporate biologics and enable uniquely high stability and biological activity. Controllable nucleation and growth of coatings also allow for spatial and temporal control over biologic delivery. In addition, coatings can be formed on a variety of tissue engineering devices, ranging from 3-D printed scaffolds to injectable microparticles. Importantly, these coatings can be independently optimized for intended biologic delivery without influencing bulk properties of the underlying device. This “modular” approach results in devices that have optimized properties from the macroscopic scale to the molecular scale. Our recent studies also demonstrate that array-based strategies can “select” coating chemistries for specific biological or biomedical goals. Examples include coatings that optimize long-term protein stabilization, autologous cell capture, stem cell differentiation, and non-viral transfection.
Bill Murphy is the Harvey D. Spangler Professor of Biomedical Engineering, Professor of Orthopedics & Rehabilitation, and Co-Director of the Stem Cell and Regenerative Medicine Center at the University of Wisconsin. He received his B.A. in Physics from Illinois Wesleyan University, Ph.D. in Biomedical Engineering from the University of Michigan, and postdoctoral training in Chemistry at the University of Chicago. His research interests focus on creating new biomaterials inspired by the materials found in nature. Murphy’s research group is using new biomaterials to understand stem cell behavior and to induce tissue regeneration. He has published more than 120 scientific manuscripts, filed over 30 patents, co-founded start-up companies, and received awards that include the National Science Foundation Career Award, the Wisconsin Vilas Associate Award, and induction as a Fellow in the American Institute for Medical and Biological Engineering.